How to optimize nuclei preparation for better genomic results

This article covers different methods for optimizing nucleus preparation. Each step of the procedure, including extraction, enrichment, counting, and purification, is recommended to ensure high-quality results.

Image Credit: DeNovix

Precisely counting nuclei and intact cells is crucial in preparing for future applications. Miltenyi Biotec researchers used a flow cytometer and a DeNovix CellDrop Automated Cell Counter to count nuclei.

Nuclei samples counted on the CellDrop (FL or FLi) were treated with Acridine Orange and Propidium Iodide fluorescent dyes to distinguish between isolated nuclei, entire cells, and debris.

The variable protocol choices and robust analysis method in CellDrop improved the accuracy of the nuclei count results. The study found that CellDrop's count results were close to those of the Miltenyi Biotec MACSQuant^™ Analyzer 10 Flow Cytometer, indicating great accuracy in both approaches.

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Award-winning products for life science

Our multi-award winning products include the Reviewers’ Choice Life Science Product of the Year and Platinum Seal awarded- DS-11 Series Spectrophotometer / Fluorometer and CellDrop^™ Automated Cell Counter. CellDrop is the first instrument of its kind to Count Cells Without Slides. These powerful instruments integrate patented DeNovix technology with easy-to-use software designed by life scientists for life scientists.

Researchers tell us they love the industry leading performance, smart-phone-like operation, and the flexible connectivity of the instruments. When support is needed, the DeNovix team is here to help. DeNovix received the prestigious Life Sciences Customer Service of the Year based on independent reviews posted by scientists worldwide!

CellDrop: Sustainable laboratory product of the year

The CellDrop Automated Cell Counter has been awarded Sustainable Laboratory Product of the Year in the SelectScience® Scientists’ Choice Awards^®!

CellDrop’s patented DirectPipette^™ technology distinguishes it as the only cell counter to eliminate the need for cell counting slides. This innovation saves millions of single-use plastic slides from use and disposal each year.

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