ATP is the main source of energy for most cellular processes. The building blocks of ATP are carbon, nitrogen, hydrogen, oxygen, and phosphorus. Because of the presence of unstable, high-energy bonds in ATP, it is readily hydrolyzed in reactions to release a large amount of energy.
The enzymatic removal of a phosphate group from ATP to form ADP releases a huge amount of energy which is used by the cell in several metabolic processes as well as in the synthesis of macromolecules such as proteins. The removal of a second phosphate group from ATP results in further energy release and the formation of adenosine monophosphate (AMP).
When energy is not needed by the organism, the phosphate group is added back to AMP and ADP to form ATP - this can be hydrolyzed later as per required. Thus, ATP functions as a reliable energy source for cellular pathways.
Functions of ATP in cells
ATP finds use in several cellular processes. Some important functions of ATP in the cell are briefly discussed below:
Active Transport
ATP plays a critical role in the transport of macromolecules such as proteins and lipids into and out of the cell. The hydrolysis of ATP provides the required energy for active transport mechanisms to carry such molecules across a concentration gradient. Transport of molecules into the cell is called endocytosis whilst transport out of the cell is known as exocytosis.
Cell Signaling
ATP has key functions both in intracellular and extracellular signaling. It is easily recognized by purinergic receptors in mammalian tissues - its release from synapses and axons activates purinergic receptors that modulate calcium and cyclic AMP levels inside the cell.
In the central nervous system, adenosine modulates neural development, the control of immune systems, and of neuron/glial signaling.
ATP is also involved in signal transduction - its phosphate groups are used up by kinases in phosphate transfer reactions which activate a cascade of protein kinase reactions.
Structural Maintenance
ATP plays a very important role in preserving the structure of the cell by helping the assembly of the cytoskeletal elements. It also supplies energy to the flagella and chromosomes to maintain their appropriate functioning.
Muscle contraction
ATP is critical for the contraction of muscles; it binds to myosin to provide energy and facilitate its binding to actin to form a cross-bridge. ADP and phosphate are then released and a new ATP molecule binds to myosin. This breaks the cross-bridge between myosin and actin filaments, thereby releasing myosin for the next contraction.
Synthesis of DNA and RNA
During DNA synthesis, ribonucleotide reductase (RNR) reduces the sugar residue from ribonucleoside diphosphates to form deoxyribonucleoside diphosphates such as dADP.
Thus, RNR regulation helps keep the balance of deoxynucleotides (dNTPs) in the cell. Low concentrations of dNTPs inhibit DNA synthesis and repair whilst high levels are shown to be mutagenic because DNA polymerase tends to add the wrong dNTP during DNA synthesis.
The adenosine from ATP is a building block of RNA and is directly added to RNA molecules during RNA synthesis by RNA polymerases. The removal of pyrophosphate provides the energy required for this reaction.
Summary
ATP is an intricate molecule that serves as an energy packet for thousands of reactions that take place in the cells of most organisms. Apart from humans, microorganisms also rely on ATP for their energy needs.
ATP is a highly efficient molecular machine with a rapid turnover of energy that makes it suitable to meet the changing energy demands of the body. An ATP molecule is over 500 atomic mass units (AMUs).
Even as evolutionists wonder about life before the complex ATP molecule and alternatives to ATP, no other energy source currently exists that can accurately respond to the energy needs of the cell and carry out its crucial processes.
References
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2877495/
- http://hyperphysics.phy-astr.gsu.edu/hbase/biology/actran.html
- www.chm.bris.ac.uk/motm/atp/atp1.htm
- http://www.trueorigin.org/atp.php
Further Reading
Assessing recurrence risk with ctDNA-based MRD detection in NSCLC patients